Nanopore 16S Amplicon Sequencing Enhances the Understanding of Pathogens in Medically Intractable Diabetic Foot Infections

Identification of Pathogens Directly From Diabetic Foot Infections by Shotgun Metagenomic Sequencing

Analysis of 16S rRNA Amplicon Sequencing Options on the Roche/454 Next-Generation Titanium Sequencing Platform

BACKGROUND 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, e...

Diabetic foot infections.

Diabetic foot infection, defined as soft tissue or bone infection below the malleoli, is the most common complication of diabetes mellitus leading to hospitalization and the most frequent cause of nontraumatic lower extremity amputation. Diabetic foot infections are diagnosed clinically based on the presence of at least two classic findings of inflammation or purulence. Infections are classifie...

Management of diabetic foot infections

Organization committee members Jean-Pierre Chambon, CHRU, Hôpital Claude-Huriez, Lille, Vascular Surgery David Elkharrat, Hôpital Ambroise-Paré, Boulogne, Emergency Room Georges Ha Van, Clinique les Trois Soleils, Boissise le Roi, Physical Medicine and Rehabilitation Agnès Heurtier-Hartemann, Hôpital de la Pitié-Salpêtrière, Paris, Endocrinology and Diabetology Fabien Koskas, Hôpital de la Piti...

Diagnosis and treatment of diabetic foot infections: adopted IWGDF guidline

Background: Diabetic foot infection is the most common complication of diabetic foot ulcer (DFU) leading to amputation. The treatment requires special attention to disease conditions, proper diagnosis, appropriate sampling for cultures, careful selection of antibiotics, rapid determination of the patient’s need for surgical intervention, and wound care. Clearly a systematic approach or, if poss...